a novel tumor suppressor reic dkk 3 gene identified by our in vitro transformation model of normal human fibroblasts works as a potent therapeutic anti tumor agent

Báo cáo khoa học: Transcriptional activity and Sp 1⁄3 transcription factor binding to the P1 promoter sequences of the human AbH-J-J locus docx

Ngày tải lên : 23/03/2014, 07:20

... ACGAACCTGTGACTCCCTCCCG TGTGTGATTTCCCGTCAACTGTC CCAGCCTCTTCCATTGGATACAA CAAGAGCAGCGGCAACAG AATAAAACTTTGGCATCATCCACTCAAAATCTCC GAACATGCCCTGGAGAAGAATGA GGTAGCCTGCATGGTCTCTTGTAT Nucleotide mutations are ... GTGCTGCAGGCGGTGCTGAGGCA TCCAGCGGCCCGCCGCCGCCAG GTGAGCAGGGGCGGGGAGCGCGGCAGGGTACCGC GTGAGCATAGTCGTAGAGCATGGTAGAGTACCGC TACACGCGAGGCCGGGCGCGCGCA CCCCCCAACACCTGCTGCCTGA ACGTTTGCCACGTTCCAAAGGA ACGAACCTGTGACTCCCTCCCG ... Lahousse SA, Califano S, Maeda T, Ueno T et al (2006) Aspartyl-(asparaginyl)beta-hydroxylase regulates hepatocellular carcinoma invasiveness J Hepatol 44, 971–9 83 11 Stamboulian S, Moutin MJ, Treves...

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Báo cáo Y học: Synthesis, conformational analysis and biological activity of cyclic analogs of the octadecaneuropeptide ODN Design of a potent endozepine antagonist pot

Ngày tải lên : 24/03/2014, 04:21

... either by a presaturation using continuous irradiation during relaxation delay or by using the gradient pulse WATERGATE [ 23] One- and two-dimensional 1H NMR spectra were calibrated using DSS as an ... experimental data with a standard dynamical simulated annealing protocol using the X -PLOR 3. 1 program A force ﬁeld adapted for NMR structure determination (ﬁle parallhdg.pro and topallhdg.pro) was ... respectively, as an agonist and an antagonist of the metabotropic receptor which mediates the ODN-induced [Ca21]i increase in rat astrocytes It has been previously shown that a vacant C-terminal carboxylic...

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Báo cáo hóa học: " Assessing agonistic potential of a candidate therapeutic anti-IL21R antibody" pptx

Ngày tải lên : 18/06/2014, 16:20

... activity assays, and project advancement to development stage ERL worked on data analysis review and manuscript preparation, MR performed the FACS based assessment of Ab-01 saturated staining in human ... "donor" + indicates that assay was performed - indicates that assay was not performed Left of/indicates RNA Right of/indicates protein To confirm that the in vitro antibody cross-linking protocols ... buffer (Qiagen, Valencia, CA) containing 1% β-mecaptoethanol Total RNA isolation was performed using the QIA Shredder and RNeasy Mini kit kits (Qiagen, Valencia, CA) according to the manufacturer's...

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Báo cáo sinh học: " Motavizumab, A Neutralizing Anti-Respiratory Syncytial Virus (Rsv) Monoclonal Antibody Significantly Modifies The Local And Systemic Cytokine Responses Induced By Rsv In The Mouse Model" pdf

Ngày tải lên : 18/06/2014, 18:20

... relative importance and role that different cytokines and chemokines play in acute RSV disease severity Instead of targeting individual cytokines as potential therapeutic targets, we took advantage ... data analyses and manuscript review, and MBR performing experiments; JC Luminex data analysis and interpretation PK and HSJ data interpretation; OR project design, experimental analyses and interpretation, ... supported in part by a Pediatric Infectious Disease Society Fellowship Award sponsored by GlaxoSmithKline Pharmaceuticals H.S.J is supported in part by the National Institute of Child Health and Human...

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báo cáo hóa học: " Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro" pot

Ngày tải lên : 19/06/2014, 22:20

... microglial activation and interleukin-1beta expression in spinal cord ischemia/reperfusion injury in rats J Thorac Cardiovasc Surg 2007, 133 (4): 934 -941 Akuzawa S, Kazui T, Shi E, Yamashita K, Bashar ... that inflammatory processes stimulated by proinflammatory cytokines and particularly by IL-1β, are rather detrimental and can aggravate the primary damage caused by infection of the CNS This has ... L, Annoni G, Davis LJ, Ferri C, Casadei V, Grimaldi LM: Increased plasma levels of interleukin-1, interleukin-6 and alpha-1-antichymotrypsin in patients with Alzheimer's disease: peripheral inflammation...

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Báo cáo hóa học: " Motavizumab, A Neutralizing Anti-Respiratory Syncytial Virus (Rsv) Monoclonal Antibody Significantly Modifies The Local And Systemic Cytokine Responses Induced By Rsv In The Mouse Model" potx

Ngày tải lên : 20/06/2014, 01:20

... relative importance and role that different cytokines and chemokines play in acute RSV disease severity Instead of targeting individual cytokines as potential therapeutic targets, we took advantage ... data analyses and manuscript review, and MBR performing experiments; JC Luminex data analysis and interpretation PK and HSJ data interpretation; OR project design, experimental analyses and interpretation, ... supported in part by a Pediatric Infectious Disease Society Fellowship Award sponsored by GlaxoSmithKline Pharmaceuticals H.S.J is supported in part by the National Institute of Child Health and Human...

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Báo cáo hóa học: " Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug " pot

Ngày tải lên : 20/06/2014, 01:20

... dUTPase as a target for antiviral therapy, F2L was deleted in the WR strain of VV and the replication of the mutant virus (VV Δ F2L-gfp) was evaluated in cell culture and in intranasally infected ... the mutant virus to some antiviral drugs A set of thymidine analogs was selected and a standard plaque reduction assay was used to evaluate the susceptibility of the dUTPase mutant and the parent ... polymerase or the spontaneous deamination of cytosine and can result in A: T transition mutations in one of the nascent strands [1] Minimizing the incorporation of these bases and excising those that...

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báo cáo hóa học:" The effect of abductor muscle and anterior-posterior hip contact load simulation on the in-vitro primary stability of a cementless hip stem" ppt

Ngày tải lên : 20/06/2014, 04:20

... 0.001° in rotation The accuracy of the system in measuring translation was evaluated against a micrometer precision dial gauge (Kafer, Germany) Translation along each of the three axes was applied ... significantly affect implant motion, and increasing the Fap to 0.6 BW only gave a significant increase in translational migration in the lateral and distal directions Park et al Journal of Orthopaedic ... hand, in another invitro study, the inclusion of muscle loads (abductor, tensor fascia latae, ilio-tibial tract, vastus lateralis and vastus medialis) increased migration and micromotion of a...

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Báo cáo y học: "Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis" pptx

Ngày tải lên : 09/08/2014, 06:23

... CCGCTATGACGCCATCTGCT R TGCACGACGAGGTCCTCACT AF019758 Decorin 55 31 9 F CAAACTCTTTTGCTTGGGCT R CACTGGACAACTCGCAGATG AF125041 Biglycan 65 204 F CCATGCTGAACGATGAGGAA R CATTATTCTGCAGGTCCAGC AF 034 842 ... Fibromodulin 65 442 F CTGGACCACAACAACCTGAC R GGATCTTCTGCAGCTGGTTG AF020291 Lumican 65 284 F CAGCCATGTACTGCGATGAG R CTGCAGGTCCACCAGAGATT NM1 739 34 TGF-β 60 271 F CGGCAGCTGTACATTGACTT R AGCGCACGATCATGTTGGAC ... GTGGGGAAACTGCACAACAT L47641 GAPDH 55 32 0 F TCACCATCTTCCAGGAGCGA R GGCGTGGACAGTGGTCATAA AF 035 421 Shown are the details of the primers used for RT-PCR, including annealing temperatures, size of the amplified...

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Báo cáo khoa học: "Radiosensitization and growth inhibition of cancer cells mediated by an scFv antibody gene against DNA-PKcs in vitro and in vivo" docx

Ngày tải lên : 09/08/2014, 09:20

... apoptosis was increased in cells expressing anti- DPK3scFv Finally, xenografts tumours expressing anti- DPK3scFv exhibited increased radiosensitivity Collectively, these data indicate that anti- DPK3-scFv ... [g -32 P] ATP As shown in Figure 4B, the activity of DNA-PK induced by Gy irradiation was greatly abolished in the HeLa-DPK3-scFv cells as compared with HeLa cells and HeLa-pcDNA cells Akt is an anti- apoptotic ... increased radiosensitivity, and inhibited DSBR In addition, DNA-PKcs associated AKT phosphorylation at S4 73 and its autophosphorylation at S2056 induced by ionizing radiation was inhibited, and apoptosis...

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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

Ngày tải lên : 09/08/2014, 14:22

... TGGAGCTTCAGAAGCTCAACACCA Antisense: ATCTCGTTGTCTGAGTACCAGTCC 51 454 IHH Sense: GAGGAGTCCCTGCATTATGA Antisense: CAGGAAAATGAGCACATCGC 54 32 1 30 RUNX2 Sense: ACAGATGATGACACTGCCACC Antisense: CATAGTAGAGATATGGAGTGCTGC ... CAGGACGACTTTGATGCAGA Antisense: AAGCTGGAGCTGTCTGGTA 54 31 2 32 FMD Sense: CTTACCCCTATGGGGTGGAT Antisense: GTACATGGCCGTGAGGAAGT 54 38 9 35 SOX9 Sense: ATCTGAAGAAGGAGAGCGAG Antisense: TCAGAAGTCTCCAGAGCTTG 58 2 63 ... markers COL II Sense: TTTCCCAGGTCAAGATGGTC Antisense: CTTCAGCACCTGTC CACCA 58 37 4 35 AGC Sense: TGAGGAGGGCTGGAACAAGTACC Antisense: GGAGGTGGTAATTGCAGGGAACA 54 39 2 30 COMP Sense: CAGGACGACTTTGATGCAGA...

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báo cáo khoa học: " Whole-Organ analysis of calcium behaviour in the developing pistil of olive (Olea europaea L.) as a tool for the determination of key events in sexual plant " ppsx

Ngày tải lên : 11/08/2014, 11:21

... developmental stages were divided into two parts, stigma with style and ovary, by using a razor blade The material was frozen and stored at -80°C Quantification of Ca2+ content Ca2+ content was measured ... controls at 575 nm was used as background The final Ca2+ amounts were calculated according to the manufacturer’sprotocol and are given in μg per μl of the sample A standard curve was prepared using ... technical assistance Author details Departamento de Bioquímica, Biolog a Celular y Molecular de Plantas, Estación Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008, Granada, Spain 2Department...

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Báo cáo y học: "Use of a highly sensitive strand-specific quantitative PCR to identify abortive replication in the mouse model of respiratory syncytial virus disease" pptx

Ngày tải lên : 12/08/2014, 01:22

... vitro standard external positive sense TCCAGCAAATACACCATCCA In vitro standard external negative sense CTGCTTCACCACCCAATTTT In vitro standard nested positive sense ATAGAATTCGGTATGTTATATGCGATGTCTAGGT1 ... ATAGAATTCGGTATGTTATATGCGATGTCTAGGT1 In vitro standard nested positive sense ATAGGATCCTGCTAAGACTCCCCACCGTAA2 Positive sense RNA-specific cDNA synthesis CGGTCATGGTGGCGAATAATCCTGCAAAAATCCCTTCAACT3 Negative sense RNA-specific cDNA ... synthesis CGGTCATGGTGGCGAATAAACTTTATAGATGTTTTTGTTCA3 Positive sense-specific QPCR primer CGGTCATGGTGGCGAATAA Probe TCCTGCAAAAATCCCTTCAACT QPCR tag primer CCCCACTTTATAGATGTTTTTGTTCA Negative sense-specific...

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Báo cáo y học: "The laminin b1-competing peptide YIGSR induces a hypercontractile, hypoproliferative airway smooth muscle phenotype in an animal model of allergic asthma" potx

Ngày tải lên : 12/08/2014, 13:22

... phenotype [14,15] Accumulation of ASM in the airway wall is a characteristic feature of asthma, which may be due to an increase in cell number (hyperplasia) [37 ,38 ] as well as an increase in cell size ... http://respiratory-research.com/content/11/1/170 Page of 11 Figure Increased ASM mass after repeated allergen challenge in vivo is inhibited by topical treatment with YIGSR To assess the role of laminins in increased ASM mass in asthma, the ... to ASM accumulation, increased expression of contractile proteins and ASM contractility, and ECM deposition are features of airway remodelling in asthma [7] In the airways of asthmatics increased...

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Báo cáo y học: "Anti-class a scavenger receptor autoantibodies from systemic lupus erythematosus patients impair phagocytic clearance of apoptotic cells by macrophages in vitro" doc

Ngày tải lên : 12/08/2014, 15:22

... new target antigens in SLE MARCO is a trimeric membrane protein containing a short N-terminal intracellular domain, a transmembrane domain, and a large extracellular domain composed of a spacer ... recombined protein was stored in small aliquots at -80°C Anti- class A scavenger receptors autoantibodies assays For the detection of anti- SRCR and anti- SR -A antibodies, high-binding plates (Costar, ... Figure 3C Association of anti- class A scavenger receptors autoantibodies with clinical features of SLE To investigate the associations between anti- class A scavenger receptors autoantibody and clinical...

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Báo cáo y học: "A mechanism of airway injury in an epithelial model of mucociliary clearance" doc

Ngày tải lên : 12/08/2014, 18:21

... repeated Injury to the palate A 50% increase in MCT was established a priori as indicative of a quantifiable injury to the mucociliary clearance system Sodium metabisulphite was applied in progressively ... have been shown to play a role in airway injury and remodeling [7,8] and in cellsignaling pathways [14] Materials and Methods Development of a frog palate injury model A fresh frog palate was ... surface The area of the palate was approximated by measuring across the lateral-most borders of the jaw at the base of the palate, and calculating the area of the equivalent half-circle The volume...

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Báo cáo y học: "Comparison of functional residual capacity and static compliance of the respiratory system during a positive end-expiratory pressure (PEEP) ramp procedure in an experimental model of acute respiratory distress syndrome" potx

Ngày tải lên : 13/08/2014, 11:22

... H2O (ARDS) after oleic acid injection During ARDS, inspiratory plateau airway pressure was significantly increased at a PEEP of 20 and 15 cm H2O (p < 0.05) Maximal oxygenation was obtained at a ... a PEEP of cm H2O (basal) Then, ARDS was induced by administration of 0.12 ml/kg of oleic acid over 30 At 120 after the beginning of oleic acid injection, a set of parameters was obtained at a ... tracheostomy tube Crs was measured by holding a 10 s inspiratory pause to obtain the value of the inspiratory plateau airway pressure (Pins) and a 10 s expiratory pause to obtain the end-expiratory...

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Báo cáo y học: " Effects of inhaled iloprost on right ventricular contractility, right ventriculo-vascular coupling and ventricular interdependence: a randomized placebo-controlled trial in an experimental model of acute pulmonary hypertension" ppt

Ngày tải lên : 13/08/2014, 11:22

... was associated with an improvement in global haemodynamics and a restoration of LV preload The reduction of RV afterload was associated with a paradoxical decrease in RV contractility Our data ... vascular resistance exhibited a nearly threefold increase from baseline conditions (Table 2) An increase in RV afterload was indicated by a higher effective PA-Ea (Figure 3a) , a lower PA compliance ... experimental time course, with a maximum pulmonary vasodilating effect immediately after inhalation and a duration of action of approximately 30 minutes The data are expressed as mean ± standard deviation...

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Báo cáo y học: "Molecular model of the outward facing state of the human P-glycoprotein (ABCB1), and comparison to a model of the human MRP5 (ABCC5)" docx

Ngày tải lên : 13/08/2014, 16:21

... ABCB9 ABCB3 ABCB2 ABCB10 ABCB8 ABCB7 ABCB6 Sav1866 ABCB4 ABCB1 ABCB5 ABCB11 ABCC12 ABCC11 ABCC5 ABCC7 ABCC4 ABCC3 ABCC1 ABCC2 ABCC6 ABCC9 ABCC8 ABCC10 ABCA7 ABCA1 ABCA4 ABCA2 ABCA3 ABCA 13 ABCA12 ABCA10 ... with blue areas indicating positive areas and red areas indicating negative areas TMHs are displayed as green ribbons TMH numbering is indicated in white boxes modeling and cGMP docking study ... ABCB, ABCC, ABCD and ABCG Two main branches are seen, with ABCB, ABCC and ABCD in one branch, and ABCA and ABCG in the other branch ABCB and ABCC subfamilies are closer related to each other than...

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Nhp6p and med3p regulate gene expression by controlling the local subunit composition of RNA polymerase II

Ngày tải lên : 11/09/2015, 16:08

... abbreviations LIST OF ABBREVIATIONS AD activating domain 6-AU 6-azauracil BD binding domain CAK cyclin-activating kinase ChIP chromatin immunoprecipitation CHL Chloramphenicol Cub C-terminal half ... 70-amino acid (domain B), and the highly acidic carboxyl-terminal 50-amino acid domain (domain C) Yeast Nhp6ap and Nhp6bp share the most identities with the middle domain and are missing the amino-third ... Nhp6p leads to increased genomic instability, hypersensitivity to DNA-damaging agents, and shortened yeast cell life span (Giavara et al., 2005) In addition, both Nhp6ap and Nhp6bp contain a highly...

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